Our systems are based on engineering of Aspergillus oryzae fungal enzyme secretion, using proteomically modified enzymes tailored for the specific waste substrate.

• A target product is identified (polypeptide, polysaccharide etc.), and computational approaches are used to locate potential secretory mechanisms.

• Candidate organisms are selected and screened, then genetically and epigenetically optimized to efficiently complete the desired valorization reaction.

• Protein structure prediction, aggregation of biochemical information and complete genome mapping allow precise targeting of enzymes to substrate, and genes to secretory mechanisms.

Leveraging this established pipeline allows rapid screening, optimization and deployment of modified A. oryzae (koji), with existing U.S. safe-use precedent and GRAS approval.